Micro-Imaging
Facility

Confocal Microscope

The LSM700 Upright confocal laser scanning microscope comprises an upright Zeiss Axio Imager Z1, four laser lines, two PMTs and a transmitted light detector (DIC compatible). The microscope is easy to use and allows high quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).

Micro Imaging Facility Support

Full training for fixed imaging
Full training for image post-processing and analysis
Fee for service

Confocal Laser Scanning Microscopy (CLSM)

CLSM uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into digital signal by the computer, producing and single-plan image. The motorized z plate-forme permits to scan through the whole specimen hence obtain and complete in-focus 3D image of the object scanned.

System Specifications

Four laser lines and transmitted light with highly sensitive detectors
Scanning resolution from 4 x 1 to 2048 x 2048 pixels
Scanning zoom from 0.5 to 40X
Motorized master pinhole with continuously adjustable diameter
Z drive for 3D imaging with increment steps down to 10 nm
Software: ZEN Black 2011

Detectors
Hamamatsu high-sensitivity compact meshless photomultiplier tubes (PMT). Spectral response ranges from 185 to 900 nm, peak wavelength: 450 nm.

Objectives