CLSM uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into digital signal by the computer, producing and single-plan image. The motorized z plate-forme permits to scan through the whole specimen hence obtain and complete in-focus 3D image of the object scanned.
The LSM700 Upright confocal laser scanning microscope comprises an upright Zeiss Axio Imager Z1, four laser lines, two PMTs and a transmitted light detector (DIC compatible). The microscope is easy to use and allows high quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).
Detectors
Hamamatsu high-sensitivity compact meshless photomultiplier tubes (PMT). Spectral response ranges from 185 to 900 nm, peak wavelength: 450 nm.
Objectives
Lasers
Classic multi-channel CLSM for fixed thin sections.
Huygens deconvolution
Dr Vanessa Lanoue
Manager
Micro Imaging Facility