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Micro-Imaging
Facility

Confocal Microscope

The LSM700 Upright confocal laser scanning microscope comprises an upright Zeiss Axio Imager Z1, four laser lines, two PMTs and a transmitted light detector (DIC compatible). The microscope is easy to use and allows high quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).

Micro Imaging Facility Support

  • Full training for fixed imaging
  • Full training for image post-processing and analysis
  • Fee for service

Confocal Laser Scanning Microscopy (CLSM)

CLSM uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into digital signal by the computer, producing and single-plan image. The motorized z plate-forme permits to scan through the whole specimen hence obtain and complete in-focus 3D image of the object scanned.


System Specifications

  • Four laser lines and transmitted light with highly sensitive detectors
  • Scanning resolution from 4 x 1 to 2048 x 2048 pixels
  • Scanning zoom from 0.5 to 40X
  • Motorized master pinhole with continuously adjustable diameter
  • Z drive for 3D imaging with increment steps down to 10 nm
  • Software: ZEN Black 2011

Detectors
Hamamatsu high-sensitivity compact meshless photomultiplier tubes (PMT). Spectral response ranges from 185 to 900 nm, peak wavelength: 450 nm.

Objectives

Lasers

Applications

Classic multi-channel CLSM for fixed thin sections.

Acquisitions post-processing

Huygens deconvolution

Enquiries

Dr Vanessa Lanoue
Manager
Micro Imaging Facility

v.lanoue@victorchang.edu.au


Discover more about the Micro Imaging Facility