Confocal Laser Scanning Microscopy (CLSM) uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into a digital signal by the computer, producing a single-plan image. The motorised z plate-form permits scanning through the whole specimen hence obtaining and completing an in-focus 3D image of the object scanned.
The LSM700 Upright confocal laser scanning microscope comprises an upright Zeiss Axio Imager Z1, four laser lines, two PMTs, and a transmitted light detector (DIC compatible). The microscope is easy to use and allows high-quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).
Hamamatsu high-sensitivity compact meshless photomultiplier tubes (PMT). Spectral response ranges from 185 to 900 nm, peak wavelength: 450 nm.
Classic multi-channel CLSM for fixed thin sections.
Huygens deconvolution
Images were acquired on a Zeiss Axio Imager.Z1 (Upright)/Observer Z1 (Inverted) fitted with an LSM 700 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step of […] nm (xy pixel size: […] nm).
When you book in time to use the Innovation Centre's Confocal Microscope, you will receive the following support from the Micro Imaging Facility team:
Dr Vanessa Lanoue, BSc, MSc, PhD
Head, Micro Imaging Facility
v.lanoue@victorchang.edu.au
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