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Confocal Microscopy

Confocal Laser Scanning Microscopy (CLSM)

Confocal Laser Scanning Microscopy (CLSM) uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into a digital signal by the computer, producing a single-plan image. The motorised z plate-form permits scanning through the whole specimen hence obtaining and completing an in-focus 3D image of the object scanned.

LSM700 Upright

The LSM700 Upright confocal laser scanning microscope comprises an upright Zeiss Axio Imager Z1, four laser lines, two PMTs, and a transmitted light detector (DIC compatible). The microscope is easy to use and allows high-quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).

LSM700 Upright System Specifications

  • Four laser lines and transmitted light with highly sensitive detectors
  • Scanning resolution from 4 x 1 to 2048 x 2048 pixels
  • Scanning zoom from 0.5 to 40X
  • Motorized master pinhole with continuously adjustable diameter
  • Z drive for 3D imaging with increment steps down to 10 nm
  • Software: ZEN Black 2011

Detectors

Hamamatsu high-sensitivity compact meshless photomultiplier tubes (PMT). Spectral response ranges from 185 to 900 nm, peak wavelength: 450 nm.

Objectives

Lasers

Applications

Classic multi-channel CLSM for fixed thin sections.

Acquisitions Post-processing

Huygens deconvolution

Citation

Images were acquired on a Zeiss Axio Imager.Z1 (Upright)/Observer Z1 (Inverted) fitted with an LSM 700 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step of […] nm (xy pixel size: […] nm).

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Confocal Microscope, you will receive the following support from the Micro Imaging Facility team:

  • Specimen preparation and mounting
  • Full training for individual or batch scanning
  • Full training for image post-processing, including reconstruction, 3D volume rendering an observation
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

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