The Freedman Foundation Metabolomics Facility
State-of-the-art liquid chromatography-mass spectrometry systems at the Victor Chang Cardiac Research Institute Innovation Centre’s Freedman Foundation Metabolomics Facility will enable researchers to measure the metabolome in a variety of samples in both targeted and untargeted modalities.
Metabolomics analysis is undertaken with the aim of measuring as much as possible of the known and unknown metabolite complement (metabolome) of a cell, tissue or biofluid. In the context of cardiovascular disease, the goal of metabolomics analysis is to discover metabolite markers that can be used to indicate or predict the onset and progression of disease, and/or the biochemical mechanisms behind it.
Based at the Victor Chang Cardiac Research Institute's new Innovation Centre, the Freedman Foundation Metabolomics Facility features:
Liquid Chromatography-Mass Spectrometry Systems
- UHPLC-Agilent 6470 QQQ
- UHPLC-Agilent 6495 QQQ
- UHPLC-SCIEX TripleTOF 6600
- UHPLC-Agilent 6560 IM-Q-TOF
The targeted instruments are used to measure several hundred known metabolites and to provide accurate concentrations, allowing changes in metabolite levels to be put into context of biochemical pathways and upstream proteome/genome perturbations.
The untargeted instruments are capable of measuring several thousand known and unknown metabolites in a single sample, with the aim of discovering new unknown compounds related to disease, or new disease-related associations of known metabolites for biomarker discovery.
![Metabolomics graphic Metabolomics graphic](https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=46&q=60&w=120 120w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=92&q=60&w=240 240w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=138&q=60&w=360 360w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=184&q=60&w=480 480w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=230&q=60&w=600 600w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=276&q=60&w=720 720w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=322&q=60&w=840 840w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=369&q=60&w=960 960w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=415&q=60&w=1080 1080w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=461&q=60&w=1200 1200w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=507&q=60&w=1320 1320w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=553&q=60&w=1440 1440w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=599&q=60&w=1560 1560w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=645&q=60&w=1680 1680w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=691&q=60&w=1800 1800w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=737&q=60&w=1920 1920w, https://victorchang-cdn.imgix.net/assets/src/uploads/Metab-3-cropped.jpg?auto=compress%2Cformat&crop=focalpoint&fit=crop&fp-x=0.5&fp-y=0.5&h=760&q=60&w=1980 1980w)
Regarded as the final part of the biological jigsaw, metabolomics can provide insight and mechanistic understanding of the pathophysiology of cardiovascular disease at the biochemical level, paving the way for predictive/diagnostic markers and ultimately, precision medicine.
Enquiries
Esther Kristianto, BSc
Research Scientist, Metabolomics Facility
e.kristianto@victorchang.edu.au
Maz Ali, BSc
Research Scientist, Metabolomics Facility
m.ali@victorchang.edu.au
Acknowledgement of Country
The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.