Nikon Eclipse Ti2E Microscope

Nikon Eclipse Ti2E Microscope

The Micro Imaging Facility's Nikon Eclipse Ti2E is a widefield fluorescent microscope ideally suited for live cell and explant imaging. Its super-fast sCMOS camera with a really large field of view, together with its different features, allows the observation of sub-millisecond events never seen before. Media perfusion and change can also be performed on this system with the high-performance microfluidics pressure and flow controller.

Nikon Eclipse Ti2E System Specifications

  • Sub-millisecond fluorescence acquisition with ultra-fast sCMOS camera2
  • CooLED fluorescent light source with microsecond switching speed for Ca2+ and visible light imaging
  • Real-time focus correction
  • Large field of view
  • Fully-equipped for live imaging, temperature and CO2 controlled
  • Elveflow multi-channel controller for high performance microfluidics pressure and flow control
  • Software: NIS Elements Advance Research

Detectors

Andor Zyla 4.2+ sCMOS camera (2048 x 2048 pixels), 6.5 μm/pixel
Frame rate from 100 fps at 2048 x 2048, up to 1570 fps at 128 x 128 pixels

Objectives

Nikon Eclipse Objective Magnification Table


CoolLED pE340fura Fluorescent Light

Applications

Live and fixed imaging

Calcium imaging, optical mapping with voltage-sensitive dye to locate and observe pacemaker and conduction pattern in explants or organoids, brightfield or fluorescent observation of ultrafast sub-millisecond events

Acquisitions post-processing

Huygens deconvolution

Citation

[Include the following citation if perfusion or incubation was used] Images were recorded from the [specimen/fluorophore] in a field of view of [… x …] mm2 with a spatial resolution of […] μm/pixel at a rate of […] frames/s on a Nikon Ti2E fitted with an Andor Zyla 4.2+ camera.

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Nikon Ti2E, you will receive the following support from the Micro Imaging Facility team:

  • Specimen preparation and mounting
  • Full training for individual or batch scanning
  • Full training for image post-processing, including reconstruction, 3D volume rendering an observation
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

Discover more instruments from the Micro Imaging Facility

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Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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