Nikon Eclipse Ti2E Microscope
Nikon Eclipse Ti2E Microscope
The Micro Imaging Facility's Nikon Eclipse Ti2E is a widefield fluorescent microscope ideally suited for live cell and explant imaging. Its super-fast sCMOS camera with a really large field of view, together with its different features, allows the observation of sub-millisecond events never seen before. Media perfusion and change can also be performed on this system with the high-performance microfluidics pressure and flow controller.
Nikon Eclipse Ti2E System Specifications
- Sub-millisecond fluorescence acquisition with ultra-fast sCMOS camera2
- CooLED fluorescent light source with microsecond switching speed for Ca2+ and visible light imaging
- Real-time focus correction
- Large field of view
- Fully-equipped for live imaging, temperature and CO2 controlled
- Elveflow multi-channel controller for high performance microfluidics pressure and flow control
- Software: NIS Elements Advance Research
Detectors
Andor Zyla 4.2+ sCMOS camera (2048 x 2048 pixels), 6.5 μm/pixel
Frame rate from 100 fps at 2048 x 2048, up to 1570 fps at 128 x 128 pixels
Objectives
Applications
Live and fixed imaging
Calcium imaging, optical mapping with voltage-sensitive dye to locate and observe pacemaker and conduction pattern in explants or organoids, brightfield or fluorescent observation of ultrafast sub-millisecond events
Acquisitions post-processing
Huygens deconvolution
Citation
[Include the following citation if perfusion or incubation was used] Images were recorded from the [specimen/fluorophore] in a field of view of [… x …] mm2 with a spatial resolution of […] μm/pixel at a rate of […] frames/s on a Nikon Ti2E fitted with an Andor Zyla 4.2+ camera.
Micro Imaging Facility Support
When you book in time to use the Innovation Centre's Nikon Ti2E, you will receive the following support from the Micro Imaging Facility team:
- Specimen preparation and mounting
- Full training for individual or batch scanning
- Full training for image post-processing, including reconstruction, 3D volume rendering an observation
- Fee for service
Enquiries
Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au
Acknowledgement of Country
The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.
