Nikon Eclipse Ti2E Microscope

Nikon Eclipse Ti2E Microscope

The Micro Imaging Facility's Nikon Eclipse Ti2E is a widefield fluorescent microscope ideally suited for live cell and explant imaging. Its super-fast sCMOS camera with a really large field of view, together with its different features, allows the observation of sub-millisecond events never seen before. Media perfusion and change can also be performed on this system with the high-performance microfluidics pressure and flow controller.

Nikon Eclipse Ti2E System Specifications

• Sub-millisecond fluorescence acquisition with ultra-fast sCMOS camera2
• CooLED fluorescent light source with microsecond switching speed for Ca2+ and visible light imaging
• Real-time focus correction
• Large field of view
• Fully-equipped for live imaging, temperature and CO2 controlled
• Elveflow multi-channel controller for high performance microfluidics pressure and flow control
• Software: NIS Elements Advance Research

Detectors

Andor Zyla 4.2+ sCMOS camera (2048 x 2048 pixels), 6.5 μm/pixel
Frame rate from 100 fps at 2048 x 2048, up to 1570 fps at 128 x 128 pixels

Objectives

Applications

Live and fixed imaging

Calcium imaging, optical mapping with voltage-sensitive dye to locate and observe pacemaker and conduction pattern in explants or organoids, brightfield or fluorescent observation of ultrafast sub-millisecond events

Acquisitions post-processing

Huygens deconvolution

Citation

[Include the following citation if perfusion or incubation was used] Images were recorded from the [specimen/fluorophore] in a field of view of [… x …] mm2 with a spatial resolution of […] μm/pixel at a rate of […] frames/s on a Nikon Ti2E fitted with an Andor Zyla 4.2+ camera.

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Nikon Ti2E, you will receive the following support from the Micro Imaging Facility team:

• Specimen preparation and mounting
• Full training for individual or batch scanning
• Full training for image post-processing, including reconstruction, 3D volume rendering an observation
• Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au