Close

Nikon Eclipse
Ti2E Microscope

Nikon Eclipse Ti2E Microscope

The Micro Imaging Facility's Nikon Eclipse Ti2E is a widefield fluorescent microscope ideally suited for live cell and explant imaging. Its super-fast sCMOS camera with a really large field of view, together with its different features, allows the observation of sub-millisecond events never seen before. Media perfusion and change can also be performed on this system with the high-performance microfluidics pressure and flow controller.

Nikon Eclipse Ti2E System Specifications

  • Sub-millisecond fluorescence acquisition with ultra-fast sCMOS camera2
  • CooLED fluorescent light source with microsecond switching speed for Ca2+ and visible light imaging
  • Real-time focus correction
  • Large field of view
  • Fully-equipped for live imaging, temperature and CO2 controlled
  • Elveflow multi-channel controller for high performance microfluidics pressure and flow control
  • Software: NIS Elements Advance Research

Detectors

Andor Zyla 4.2+ sCMOS camera (2048 x 2048 pixels), 6.5 μm/pixel
Frame rate from 100 fps at 2048 x 2048, up to 1570 fps at 128 x 128 pixels

Objectives

Nikon Eclipse Objective Magnification Table


CoolLED pE340fura Fluorescent Light

Applications

Live and fixed imaging

Calcium imaging, optical mapping with voltage-sensitive dye to locate and observe pacemaker and conduction pattern in explants or organoids, brightfield or fluorescent observation of ultrafast sub-millisecond events

Acquisitions post-processing

Huygens deconvolution

Citation

Include the following citation if perfusion or incubation was used:

Images were recorded from the [specimen/fluorophore] in a field of view of [… x …] mm2 with a spatial resolution of […] μm/pixel at a rate of […] frames/s on a Nikon Ti2E fitted with an Andor Zyla 4.2+ camera.

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Nikon Ti2E, you will receive the following support from the Micro Imaging Facility team:

  • Specimen preparation and mounting
  • Full training for individual or batch scanning
  • Full training for image post-processing, including reconstruction, 3D volume rendering an observation
  • Fee for service

Enquiries

Dr Vanessa Lanoue, BSc, MSc, PhD
Head, Micro Imaging Facility
v.lanoue@victorchang.edu.au

Discover more instruments from the Micro Imaging Facility

Visit other Innovation Centre Facilities