`
Close

Micro-Imaging
Facility

Nikon Eclipse Ti2E Microscope

The Nikon Eclipse Ti2E is a widefield fluorescent microscope ideally suited for live cell and explant imaging. Its super-fast sCMOS camera with a really large field of view, together with its different features, allow the observation of sub-millisecond events never seen before. Media perfusion and change can also be performed on this system with the high-performance microfluidics pressure and flow controller.

MIF Support

Full training for fixed and live imaging
Full training for image/time-lapse postprocessing and analysis
Fee for service

System Specifications

Sub-millisecond fluorescence acquisition with ultra-fast sCMOS camera2>
CooLED fluorescent light source with microsecond switching speed for Ca2+ and visible light imaging
Real-time focus correction
Large field of view
Fully equipped for live imaging, temperature and CO2 controlled
Elveflow multi-channel controller for high performance microfluidics pressure and flow control
Software: NIS Elements Advance Research

Detectors

Andor Zyla 4.2+ sCMOS camera (2048 x 2048 pixels), 6.5 μm/pixel
Frame rate from 100 fps at 2048 x 2048, up to 1570 fps at 128 x 128 pixels

Objectives

Nikon Eclipse Objective Magnification Table


CoolLED pE340fura Fluorescent Light


Applications

Live and fixed imaging

Calcium imaging, optical mapping with voltage-sensitive dye to locate and observe pacemaker and conduction pattern in explants or organoids, brightfield or fluorescent observation of ultrafast sub-millisecond events

Acquisitions post-processing

Huygens deconvolution

Citation

[Include here if perfusion or incubation was used] Images were recorded from the [specimen/fluorophore] in a field of view of [… x …] mm2 with a spatial resolution of […] μm/pixel at a rate of […] frames/s on a Nikon Ti2E fitted with an Andor Zyla 4.2+ camera.

Enquiries

Dr Vanessa Lanoue
Manager
Micro Imaging Facility

v.lanoue@victorchang.edu.au


Discover more about the Micro Imaging Facility