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PerkinElmer Opera Phenix

Opera Phenix

The PerkinElmer Opera Phenix is a high content screening system that allows high throughput imaging and analysis of hundreds of conditions. The system, fitted with a multi-well plate holder or a 4-slide holder can be used to image in brightfield and digital phase contrast, and/or multi-channel widefield fluorescence or spinning disk confocal. Its sealed chamber permits both fixed and live-cell imaging.

Opera Phenix

System Specifications


2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2160 x 2160 pixels)


Opera Phenix magnification table


Light Sheet Lasers Table


Live and fixed imaging
Live- or fixed-cell assays, 3D cell culture, non-labelled or labelled specimens, small organisms (zebrafish larvae), organoids.

Opera Phenix - Live and fixed imaging" title="Opera Phenix - Innovation Centre


Workflow-based automated image analysis with Harmony software. Hundreds of measurements available, including 2D and 3D analyses, cell counting, cell classification, morphology analysis, intensity measurements, and colocalization. Optional data export for Cell Profiler- or ImageJ-based image analysis and/or Huygens deconvolution.


Images were acquired on a PerkinElmer Opera Phenix microscope with [precise objective, channels, time-lapse used]. Analyses were performed with Harmony 4.9 software using [precise pipeline designed].

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Opera Phenix, you will receive the following support from the Micro Imaging Facility team:


Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility

Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.

Victor Chang Cardiac Research Institute - The Home of Heart Research for 30 Years