PerkinElmer Opera Phenix
Opera Phenix
The PerkinElmer Opera Phenix is a high content screening system that allows high throughput imaging and analysis of hundreds of conditions. The system, fitted with a multi-well plate holder or a 4-slide holder can be used to image in brightfield and digital phase contrast, and/or multi-channel widefield fluorescence or spinning disk confocal. Its sealed chamber permits both fixed and live-cell imaging.
System Specifications
- Dual high sensitivity camera configuration
- Spinning disk with increased pinhole-to-pinhole spacing to reduce out-of-focus noise and fluorescence cross talk
- Large field of view
- Laser-based autofocus
- Fully equipped for live imaging, temperature and CO2 control
- Plate Wizard in the software to enter specific plate dimensions (fits plates from 6- to 1536-well)
- Software: Harmony 4.9
Detectors
2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2160 x 2160 pixels)
Objectives
Lasers
Applications
Live and fixed imaging
Live- or fixed-cell assays, 3D cell culture, non-labelled or labelled specimens, small organisms (zebrafish larvae), organoids.
Analysis
Workflow-based automated image analysis with Harmony software. Hundreds of measurements available, including 2D and 3D analyses, cell counting, cell classification, morphology analysis, intensity measurements, and colocalization. Optional data export for Cell Profiler- or ImageJ-based image analysis and/or Huygens deconvolution.
Citation
Images were acquired on a PerkinElmer Opera Phenix microscope with [precise objective, channels, time-lapse used]. Analyses were performed with Harmony 4.9 software using [precise pipeline designed].
Micro Imaging Facility Support
When you book in time to use the Innovation Centre's Opera Phenix, you will receive the following support from the Micro Imaging Facility team:
- PerkinElmer multi-well plates available to buy from the facility
- Training for fixed and live imaging
- Training for image analysis within Harmony 4.9, or help available for image analysis in alternative software
- Fee for service
Enquiries
Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au
Acknowledgement of Country
The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.
