Opera Phenix

The PerkinElmer Opera Phenix is a high content screening system that allows high throughput imaging and analysis of hundreds of conditions at the fastest speed currently possible. The system, fitted with a multiwell plate holder or a 4-slide holder can be used to image in brightfield and digital phase contrast, and/or multi-channel fluorescent widefield or spinning disc confocal. Its sealed chamber permits both fixed and live-cell imaging.

System Specifications

• Dual high sensitivity camera configuration
• Spinning disk with increased pinhole-to-pinhole spacing to reduce out-of-focus noise and fluorescence cross talk
• Large field of view
• Laser-based autofocus
• Fully equipped for live imaging, temperature and CO2 controlled
• Plate Wizard in the software to enter specific plate dimension (fits plates from 6- to 1536-well)
• Software: Harmony 4.8

Detectors

2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2100 x 2100 pixels)

Objectives

Lasers

Applications

Live and fixed imaging
Live-or fixed-cell assays, 3D cell culture, non-labelled or labelled specimen, small organisms (zebrafish larvae), organoids

Analysis

Cell counting, morphology analysis, intensity measurements, colocalization.

Acquisitions post-processing

Harmony-, CellProfiler- or ImageJ-based cell counting and further analysis, Huygens deconvolution

Citation

Images were acquired on a PerkinElmer Opera Phenix microscope with [precise objective, channels, time-lapse used]. Analysis were performed with the Harmony software using [precise pipeline designed].

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Opera Phenix, you will receive the following support from the Micro Imaging Facility team:

• PerkinElmer multiwell plates available to buy from the MIF
• Full training for fixed and live imaging
• Full training for image analysis within Harmony 4.8, or help provided for ImageJ or CellProfiler based analysis
• Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

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