The PerkinElmer Opera Phenix is a high content screening system that allows high throughput imaging and analysis of hundreds of conditions at the fastest speed currently possible. The system, fitted with a multiwell plate holder or a 4-slide holder can be used to image in brightfield and digital phase contrast, and/or multi-channel fluorescent widefield or spinning disc confocal. Its sealed chamber permits both fixed and live-cell imaging.
2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2100 x 2100 pixels)
Live and fixed imaging
Live-or fixed-cell assays, 3D cell culture, non-labelled or labelled specimen, small organisms (zebrafish larvae), organoids
Cell counting, morphology analysis, intensity measurements, colocalization.
Harmony-, CellProfiler- or ImageJ-based cell counting and further analysis, Huygens deconvolution
Images were acquired on a PerkinElmer Opera Phenix microscope with [precise objective, channels, time-lapse used]. Analysis were performed with the Harmony software using [precise pipeline designed].
When you book in time to use the Innovation Centre's Opera Phenix, you will receive the following support from the Micro Imaging Facility team:
Dr Vanessa Lanoue, BSc, MSc, PhD
Head, Micro Imaging Facility
v.lanoue@victorchang.edu.au
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