PerkinElmer Opera Phenix

Opera Phenix

The PerkinElmer Opera Phenix is a high content screening system that allows high throughput imaging and analysis of hundreds of conditions at the fastest speed currently possible. The system, fitted with a multiwell plate holder or a 4-slide holder can be used to image in brightfield and digital phase contrast, and/or multi-channel fluorescent widefield or spinning disc confocal. Its sealed chamber permits both fixed and live-cell imaging.

System Specifications

  • Dual high sensitivity camera configuration
  • Spinning disk with increased pinhole-to-pinhole spacing to reduce out-of-focus noise and fluorescence cross talk
  • Large field of view
  • Laser-based autofocus
  • Fully equipped for live imaging, temperature and CO2 controlled
  • Plate Wizard in the software to enter specific plate dimension (fits plates from 6- to 1536-well)
  • Software: Harmony 4.8

Detectors

2x 16-bit sCMOS cameras for simultaneous dual colour acquisition (2100 x 2100 pixels)

Objectives

Lasers

Applications

Live and fixed imaging
Live-or fixed-cell assays, 3D cell culture, non-labelled or labelled specimen, small organisms (zebrafish larvae), organoids

Opera Phenix - Innovation Centre

Analysis

Cell counting, morphology analysis, intensity measurements, colocalization.

Acquisitions post-processing

Harmony-, CellProfiler- or ImageJ-based cell counting and further analysis, Huygens deconvolution

Citation

Images were acquired on a PerkinElmer Opera Phenix microscope with [precise objective, channels, time-lapse used]. Analysis were performed with the Harmony software using [precise pipeline designed].

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Opera Phenix, you will receive the following support from the Micro Imaging Facility team:

  • PerkinElmer multiwell plates available to buy from the MIF
  • Full training for fixed and live imaging
  • Full training for image analysis within Harmony 4.8, or help provided for ImageJ or CellProfiler based analysis
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

Discover more instruments from the Micro Imaging Facility

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Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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