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Optics11 Chiaro Nanoindenter

Chiaro Nanoindenter

The Optics11 Chiaro is the only Nanoindenter in Australia. It measures force and displacement to investigate the micro-mechanical properties of biomaterials ranging in stiffness between 10 Pa and 1 GPa. These include single cells, native or engineered tissues, spheroids/organoids, hydrogels, biofilms and other non-organic specimens.

The Chiaro is combined to the LSM700 Inverted Confocal Microscope, allowing high-resolution imaging of mechanically characterized material which enables structure-stiffness analysis or more advanced mechanobiology experiments such as mechanically stimulate cells to induce calcium waves or measure cardiac cell beating amplitude.

Scientist experimenting with Chiaro Nanoindenter, a close up look of Chiaro Nanoindenter

System Specifications

Chiaro Nanoindenter


See LSM700 Inverted Confocal Microscope

Technical Specifications

Chiaro Nanoindenter Specifications Table


Assess stiffness and viscoelastic properties of biological or inorganic specimens to compare mechanical properties. For example;

Acquisitions post-processing

DataViewer analysis


Stiffness/viscoelasticity of [specimen] was tested using a CHIARO nanoindenter system (Optics11, Amsterdam, The Netherlands). A colloidal probe with a spring constant of [x] N/m and a diameter of [n] μm was used. Before testing, the sensitivity calibration of the cantilever was conducted by indenting a hard surface (i.e. a glass Petri dish).

Micro Imaging Facility Support


Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility

Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.

Victor Chang Cardiac Research Institute - The Home of Heart Research for 30 Years