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Optics11 Chiaro Nanoindenter

Chiaro Nanoindenter

The Optics11 Chiaro is the only Nanoindenter in Australia. It measures force and displacement to investigate the micro-mechanical properties of biomaterials ranging in stiffness between 10 Pa and 1 GPa. These include single cells, native or engineered tissues, spheroids/organoids, hydrogels, biofilms and other non-organic specimens.

The Chiaro is combined to the LSM700 Inverted Confocal Microscope, allowing high-resolution imaging of mechanically characterized material which enables structure-stiffness analysis or more advanced mechanobiology experiments such as mechanically stimulate cells to induce calcium waves or measure cardiac cell beating amplitude.

Scientist experimenting with Chiaro Nanoindenter, a close up look of Chiaro Nanoindenter

System Specifications

Chiaro Nanoindenter

Confocal

See LSM700 Inverted Confocal Microscope

Technical Specifications

Chiaro Nanoindenter Specifications Table

Applications

Assess stiffness and viscoelastic properties of biological or inorganic specimens to compare mechanical properties. For example;

Acquisitions post-processing

DataViewer analysis

Citation

Stiffness/viscoelasticity of [specimen] was tested using a CHIARO nanoindenter system (Optics11, Amsterdam, The Netherlands). A colloidal probe with a spring constant of [x] N/m and a diameter of [n] μm was used. Before testing, the sensitivity calibration of the cantilever was conducted by indenting a hard surface (i.e. a glass Petri dish).

Micro Imaging Facility Support

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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