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Confocal Microscopy

Confocal Laser Scanning Microscopy (CLSM)

Confocal Laser Scanning Microscopy (CLSM) uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into a digital signal by the computer, producing a single-plan image. The motorised z plate-form permits scanning through the whole specimen hence obtaining and completing an in-focus 3D image of the object scanned.

Confocal Microscopes available in our Innovation Centre:

Micro Imaging Facility Support

When you book in time to use the Innovation Centre's Confocal Microscopes, you will receive the following support from the Micro Imaging Facility team with:

  • Specimen preparation and mounting
  • Full training for individual or batch scanning
  • Full training for image post-processing, including reconstruction, 3D volume rendering an observation
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

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Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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