Confocal Microscopy
Confocal Laser Scanning Microscopy (CLSM)
Confocal Laser Scanning Microscopy (CLSM) uses a laser beam to illuminate a small area of the specimen. The emitted light passes through a pinhole, allowing only the in-focus light to be detected. The faint light emitted is amplified by the photomultipliers that generate an electrical signal further converted into a digital signal by the computer, producing a single-plan image. The motorised z plate-form permits scanning through the whole specimen hence obtaining and completing an in-focus 3D image of the object scanned.
Confocal Microscopes available in our Innovation Centre:
Micro Imaging Facility Support
When you book in time to use the Innovation Centre's Confocal Microscopes, you will receive the following support from the Micro Imaging Facility team with:
- Specimen preparation and mounting
- Full training for individual or batch scanning
- Full training for image post-processing, including reconstruction, 3D volume rendering an observation
- Fee for service
Enquiries
Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au
Acknowledgement of Country
The Victor Chang Cardiac Research Institute acknowledges Traditional Owners of Country throughout Australia and recognises the continuing connection to lands, waters and communities. We pay our respect to Aboriginal and Torres Strait Islander cultures; and to Elders past and present.
