Confocal Microscopy

LSM900 Inverted

The Zeiss LSM900 Inverted confocal laser scanning microscope comprises an inverted Zeiss Axio Observer 7, four laser lines, two GaAsP-PMT detectors, and a motorised stage. The microscope is easy to use and allows high-quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).

LSM900 Microscope | Innovation Centre
Confocal Microscope Usage Sample

LSM900 Inverted System Specifications

  • Four laser lines with highly sensitive GaAsP-PMT detectors
  • Scanning resolution from 4 x 1 to 6144 x 6144 pixels
  • Scanning zoom from 0.5 to 40X
  • Motorized master pinhole with continuously adjustable diameter
  • Motorised focus drive for 3D imaging with increment steps down to 10 nm
  • Motorised scanning stage with 1 um reproducibility
  • Colibri 5 solid state LED fluorescence light source
  • Differential Interference Contrast (DIC) optics
  • Software: ZEN blue 3.4

Detectors

Two Gallium Arsenide Phosphide photomultiplier tubes (GaAsP-PMT). Typical maximum Quantum Efficiency: 45%.

Objectives

LSM900 Objectives Table | Innovation Centre

Lasers

LSM900 Lasers Specifications Table | Innovation Centre

Applications

  • Classic multi-channel CLSM for fixed thin sections
  • Z-stack 3D imaging
  • Tiled imaging of large specimens

Citation

Images were acquired on a Zeiss Axio Observer 7 fitted with an LSM 900 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step size of […] nm (xy pixel size: […] nm).

Micro Imaging Facility Support

Support from the Micro Imaging Facility team is available for the Innovation Centre's LSM900 Inverted Confocal Microscope, including:

  • Training for instrument usage
  • Training and advice for image processing and analysis
  • Advice and consumables for specimen preparation and mounting
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

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Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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