Confocal Microscopy

LSM900 Inverted

The Zeiss LSM900 Inverted confocal laser scanning microscope comprises an inverted Zeiss Axio Observer 7, four laser lines, two GaAsP-PMT detectors, and a motorised stage. The microscope is easy to use and allows high-quality multicolour imaging, thus perfect for classic laser scanning confocal microscopy (CLSM).

LSM900 Inverted System Specifications

• Four laser lines with highly sensitive GaAsP-PMT detectors
• Scanning resolution from 4 x 1 to 6144 x 6144 pixels
• Scanning zoom from 0.5 to 40X
• Motorized master pinhole with continuously adjustable diameter
• Motorised focus drive for 3D imaging with increment steps down to 10 nm
• Motorised scanning stage with 1 um reproducibility
• Colibri 5 solid state LED fluorescence light source
• Differential Interference Contrast (DIC) optics
• Software: ZEN blue 3.4

Detectors

Two Gallium Arsenide Phosphide photomultiplier tubes (GaAsP-PMT). Typical maximum Quantum Efficiency: 45%.

Objectives

Lasers

Applications

• Classic multi-channel CLSM for fixed thin sections
• Z-stack 3D imaging
• Tiled imaging of large specimens

Citation

Images were acquired on a Zeiss Axio Observer 7 fitted with an LSM 900 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step size of […] nm (xy pixel size: […] nm).

Micro Imaging Facility Support

Support from the Micro Imaging Facility team is available for the Innovation Centre's LSM900 Inverted Confocal Microscope, including:

• Training for instrument usage
• Training and advice for image processing and analysis
• Advice and consumables for specimen preparation and mounting
• Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au