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Confocal Microscopy

LSM7DUO (LSM710) Inverted

The Zeiss LSM7DUO (LSM710) Inverted confocal laser scanning microscope comprises an inverted Zeiss Axio Observer.Z1, seven laser lines, 34-channel QUASAR PMT detectors, and a motorised stage. The microscope supports time-lapse live cell confocal imaging with temperature and atmosphere control, Z-stack 3D imaging and tiled imaging of large specimens

LSM7DUO (LSM710) Inverted Confocal Microscope | Innovation Centre
Microscopic image captured using confocal microscopy

LSM7DUO (LSM710) Inverted System Specifications

  • Seven laser lines and transmitted light with highly sensitive detectors
  • Scanning resolution from 4 x 1 to 6144 x 6144 pixels
  • Scanning zoom from 0.6 to 40X
  • Motorized master pinhole with continuously adjustable diameter
  • Z drive for 3D imaging with increment steps down to 10 nm
  • Temperature, CO2 and O2 level regulation for live cell imaging
  • Software: ZEN Black 2012 SP1

Detectors

34-channel QUASAR spectral detection with highly sensitive low dark noise PMTs. Spectral detection range freely selectable (resolution down to 3 nm). Transmitted light PMT.

Objectives

LSM7 Duo Confocal Microscope Objectives

Lasers

LSM7DUO Lasers Confocal Microscopy

Applications

  • Classic multi-channel CLSM for fixed thin sections
  • Live cell imaging
  • Z-stack 3D imaging
  • Tiled imaging of large specimens
  • Photobleaching/FRAP and photoactivation

Citation

Images were acquired on a Zeiss Observer.Z1 fitted with an LSM 710 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step of […] nm (xy pixel size: […] nm).

Micro Imaging Facility Support

Support from the Micro Imaging Facility team is available for the Innovation Centre's LSM7DUO (LSM710) Inverted Confocal Microscope, including:

  • Training for instrument usage
  • Training and advice for image processing and analysis
  • Advice and consumables for specimen preparation and mounting
  • Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au

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Acknowledgement of Country

The Victor Chang Cardiac Research Institute acknowledges the traditional custodians of the land, the Gadigal of the Eora nation, on which we meet, work, and discover.
Our Western Australian laboratories pay their respect to the Whadjuk Noongar who remain as the spiritual and cultural custodians of their land.

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