Confocal Microscopy

LSM7DUO (LSM710) Inverted

The Zeiss LSM7DUO (LSM710) Inverted confocal laser scanning microscope comprises an inverted Zeiss Axio Observer.Z1, seven laser lines, 34-channel QUASAR PMT detectors, and a motorised stage. The microscope supports time-lapse live cell confocal imaging with temperature and atmosphere control, Z-stack 3D imaging and tiled imaging of large specimens

LSM7DUO (LSM710) Inverted System Specifications

• Seven laser lines and transmitted light with highly sensitive detectors
• Scanning resolution from 4 x 1 to 6144 x 6144 pixels
• Scanning zoom from 0.6 to 40X
• Motorized master pinhole with continuously adjustable diameter
• Z drive for 3D imaging with increment steps down to 10 nm
• Temperature, CO₂ and O₂ level regulation for live cell imaging
• Software: ZEN Black 2012 SP1

Detectors

34-channel QUASAR spectral detection with highly sensitive low dark noise PMTs. Spectral detection range freely selectable (resolution down to 3 nm). Transmitted light PMT.

Objectives

Lasers

Applications

• Classic multi-channel CLSM for fixed thin sections
• Live cell imaging
• Z-stack 3D imaging
• Tiled imaging of large specimens
• Photobleaching/FRAP and photoactivation

Citation

Images were acquired on a Zeiss Observer.Z1 fitted with an LSM 710 confocal scan head, using a […]X objective, numerical aperture: […], with a z-step of […] nm (xy pixel size: […] nm).

Micro Imaging Facility Support

Support from the Micro Imaging Facility team is available for the Innovation Centre's LSM7DUO (LSM710) Inverted Confocal Microscope, including:

• Training for instrument usage
• Training and advice for image processing and analysis
• Advice and consumables for specimen preparation and mounting
• Fee for service

Enquiries

Dr Scott Page, BSc, PhD
Head, Micro Imaging Facility
s.page@victorchang.edu.au